Disruption of the CAR1 gene encoding arginase enhances freeze tolerance of the commercial baker's yeast Saccharomyces cerevisiae.

نویسندگان

  • Jun Shima
  • Yuko Sakata-Tsuda
  • Yasuo Suzuki
  • Ryouichi Nakajima
  • Hajime Watanabe
  • Shinichi Kawamoto
  • Hiroyuki Takano
چکیده

The effect of intracellular charged amino acids on freeze tolerance in dough was determined by constructing homozygous diploid arginase-deficient mutants of commercial baker's yeast. An arginase mutant accumulated higher levels of arginine and/or glutamate and showed increased leavening ability during the frozen-dough baking process, suggesting that disruption of the CAR1 gene enhances freeze tolerance.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 69 1  شماره 

صفحات  -

تاریخ انتشار 2003